產品英文名稱：Mouse Total IgG ELISA kit
產品中文名稱：小鼠總 IgG 抗體 ELISA 試劑盒

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Mouse Total IgG ELISA kit 的應用
本試劑盒是用于定量小鼠總的IgG抗體。本試劑盒使用的抗體對小鼠免疫球蛋白是特異性的并且不與來自其他物種的免疫球蛋白交叉反應。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。

Mouse Total IgG ELISA kit 的英文描述
The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulins from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. All of the Mouse Immunoglobulin ELISA kits can also be used to quantitate mouse immunoglobulin levels in serum of other animals administered mouse monoclonal antibodies in research studies.
Monoclonal antibodies are used in many applications such asdiagnostic or therapeutic agents as well as affinity supports for immunoaffinity purification of various products. These applications frequently require a sensitive assay to accuray quantitate the concentration of these antibodies in complex sample matrices. The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulin from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. The presence of high concentrations of mouse IgM and IgA immunoglobulin types in the sample can interfere in the ability of
this kit to quantitate mouse IgG. Cygnus Technologies provides kits for IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM immunoglobulins. See ordering information on the last page.

小鼠總 IgG 抗體 ELISA 試劑盒的使用步驟規(guī)則
The Mouse IgG assay is a two-site immunoenzymetric assay. Samples containing mouse IgG are reacted in microtiter strips coated with an affinity purified capture antibody. A second alkaline phosphatase enzyme labeled goat anti-mouse immmunoglobulin antibody is reacted, forming a sandwich
complex of solid phase antibody-mouse immunoglobulinenzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly
proportional to the concentration of mouse IgG present. Accurate quantitation is achieved by comparing the signal of unknowns to IgG standards assayed at the same time.

Mouse Total IgG ELISA kit 的保存和穩(wěn)定性
1 All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
2 The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
3 Reconstituted wash solution is stable until the expiration date of the kit.

小鼠總 IgG抗體ELISA試劑盒的使用注意事項
1 只用于研究或制造。
2 在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
3 本試劑盒只能由合格的技術人員使用。

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